Barclays Bank is shutting its branch at the Asda Broadstairs store.
The service will shut on January 18 next year due to a lack of customers.
Barclays says there are only 159 customers who do all their banking at the branch and the majority of customers use alternative services, such as online or telephone banking.
The announcement that Broadstairs branch would open in the Asda supermarket was made in 2013 following the loss of the High Street service.
It is understood that six staff at the branch are affected.
A Barclays spokesman confirmed there will not be any redundancies.
The closure means residents in Broadstairs will only have Nationwide in the High Street and the HSBC at Westwood Cross for face to face services unless they travel to other towns.
The nearest Barclays branches will be Ramsgate, Margate and Deal.
A Barclays statement says: “Our branch network and the colleagues who work in them remain a vital part of what we offer our customers.
“Yet with customers visiting our branches less and less each year, we must constantly assess how and why our branches are used and make decisions based on that insight. There will be times when this means that we will make the difficult decision to close a branch.”